I have never done a stack over 100 and my poor focus rail would hardly allow that many advances on something this size. I would suggest making your first attempt something a great deal smaller (maybe 20) shot at a smaller aperture so there is more overlap. The automated focus bracketing feature available on all RF bodies except the original R is easier to use but limited to things not so extreme. I get better results doing manual advances when beyond 1:1. There are many uses for focus bracketing in landscapes where you really need only 2 or three shots but you get to a point in the extreme macro where you will be unhappy that you got fuzzy gaps so you shoot many. I usually end up shooting more than I think I'll need and then elimination the ones that contribute nothing when I stack them. Start small and simple; work up to the extreme ant portrait stage (I'm not there yet). Flowers are a good place to start. This Crocosmia used the automated feature on my RP with the old version EF 100 macro. Unfortunately, I rarely record such details as how many shots or what f/stop was used and the EXIF data does not survive the DPP4 stacking routine (or I don't know how to enable it?).
I have never done a stack over 100 and my poor focus rail would hardly allow that many advances on something this size. I would suggest making your first attempt something a great deal smaller (maybe 20) shot at a smaller aperture so there is more overlap. The automated focus bracketing feature available on all RF bodies except the original R is easier to use but limited to things not so extreme. I get better results doing manual advances when beyond 1:1. There are many uses for focus bracketing in landscapes where you really need only 2 or three shots but you get to a point in the extreme macro where you will be unhappy that you got fuzzy gaps so you shoot many. I usually end up shooting more than I think I'll need and then elimination the ones that contribute nothing when I stack them. Start small and simple; work up to the extreme ant portrait stage (I'm not there yet). Flowers are a good place to start. This Crocosmia used the automated feature on my RP with the old version EF 100 macro. Unfortunately, I rarely record such details as how many shots or what f/stop was used and the EXIF data does not survive the DPP4 stacking routine (or I don't know how to enable it?).
dougsmit, All good advice. I use a manual rail and it is quite tedious. There may be an auto rail in my future if I do too many more of these. As far as using the in camera focus bracketing in camera on the Canon R* bodies I've had great luck with that feature. I used my EF 100mm f/2.8 macro on a cicada the other day that it did a great job with focus bracketing on 200 slices. I didn't like the final image because the cicada had been dead a while and the ants had gotten to it, but here's how it turned out. Of course this one is cropped in quite a bit here but the in camera focus bracket did a great job.